#30: Creating an Enzyme-based Fluorogenic Reporter for Potato Virus Y — Presentation Detail — Celebration of Scholars

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Student presentations must have a faculty sponsor.

Abstracts must include a title and a description of the research, scholarship, or creative work. The description should be 150-225 words in length and constructed in a format or style appropriate for the presenter’s discipline.

The following points should be addressed within the selected format or style for the abstract:

A clear statement of the problem or question you pursued, or the scholarly goal or creative theme achieved in your work.
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Additional Information

More information is available at carthage.edu/celebration-scholars/. The following are members of the Research, Scholarship, and Creativity Committee who are eager to listen to ideas and answer questions:

Jun Wang
Kim Instenes
John Kirk
Nora Nickels
Andrew Pustina
James Ripley

#30: Creating an Enzyme-based Fluorogenic Reporter for Potato Virus Y

Name: Damian Leja
Major: Biology
Hometown: Burbank, IL
Faculty Sponsor:
Other Sponsors:
Type of research: SURE
Funding: SURE

Abstract

Potato Virus Y (PVY) is a prevalent pathogenic virus that infects many potato cultivars globally and leads to decreased crop yields. The symptom presentation of an infected plant varies greatly depending on the cultivar, making visual detection of an infected crop difficult. Currently, an enzyme-linked immunosorbent assay (ELISA) is used to detect the presence of PVY. However, this method requires a high viral load, attainable 2-3 weeks after infection, at which point the plant is fully infected. The project aims to develop a fluorogenic detection system made up of two modified constructs of green fluorescent protein (GFP) activated by the N1A protease, which is required for PVY replication. Upon activation, the parts reconstitute a functional GFP, resulting in a fluorescent signal. I attempted to insert DNA fragments that encoded the constructs into the one Duet plasmid with two insertion sites, one for each construct.

Poster file