#44: Spotlight on Light Sheet Fluorescence Microscopy — Presentation Detail — Celebration of Scholars

Instructions

Student presentations must have a faculty sponsor.

Abstracts must include a title and a description of the research, scholarship, or creative work. The description should be 150-225 words in length and constructed in a format or style appropriate for the presenter’s discipline.

The following points should be addressed within the selected format or style for the abstract:

A clear statement of the problem or question you pursued, or the scholarly goal or creative theme achieved in your work.
A brief comment about the significance or uniqueness of the work.
A clear description of the methods used to achieve the purpose or goals for the work.
A statement of the conclusions, results, outcomes, or recommendations, or if the work is still in progress, the results you expect to report at the event.

Presenter photographs should be head and shoulder shots comparable to passport photos.

Additional Information

More information is available at carthage.edu/celebration-scholars/. The following are members of the Research, Scholarship, and Creativity Committee who are eager to listen to ideas and answer questions:

Jun Wang
Kim Instenes
John Kirk
Nora Nickels
Andrew Pustina
James Ripley

#44: Spotlight on Light Sheet Fluorescence Microscopy

Name: Rebecca Dowe
Major: Physics
Hometown: Port Washington, WI
Faculty Sponsor:
Other Sponsors:
Type of research: SURE

Abstract

Within the last decade, light sheet fluorescence microscopy (LSFM) has gained traction as a method of imaging biological specimens. The microscope’s laser light is formed into a thin sheet, so that only a single plane of the specimen is illuminated at a time. Every plane is combined into a full 3D image, which can then be analyzed. LSFM is gaining traction primarily because of its low phototoxicity, due to single-plane illumination, meaning it is significantly less harmful for the specimen compared to other imaging methods. After comparing a few options for software to run the microscope, I opted for Micro-Manager because of its simplicity and available resources, and began configuring the hardware. A triggerscope serves as communication between the hardware and the computer, which optimizes the speed of imaging. The process of configuring and optimizing the hardware with the software is ongoing. In the future, the microscope will be used to study development of the zebrafish visual system.

Poster file