#38: Fluoro-Gold Tracing in Various Brain Regions of Sprague-Dawley Rats — Presentation Detail — Celebration of Scholars

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More information is available at carthage.edu/celebration-scholars/. The following are members of the Research, Scholarship, and Creativity Committee who are eager to listen to ideas and answer questions:

Jun Wang
Kim Instenes
John Kirk
Nora Nickels
Andrew Pustina
James Ripley

#38: Fluoro-Gold Tracing in Various Brain Regions of Sprague-Dawley Rats

Name: Frannie Drake
Major: Neuroscience
Hometown: Naperville, IL
Faculty Sponsor:
Other Sponsors:
Type of research: Course project

Name: Alexis Cabell
Major: Neuroscience
Hometown: Grafton, WI
Faculty Sponsor:
Other Sponsors:
Type of research: Course project

Name: Kristie Endresen
Major: Neuroscience
Hometown: East Troy, WI
Faculty Sponsor:
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Type of research: Course project

Name: Amber Janke
Major: Neuroscience, Psychology, and Data Science
Hometown: Mount Pleasant, WI
Faculty Sponsor:
Other Sponsors:
Type of research: Course project

Name: Allyson Lengers
Major: Neuroscience
Hometown: White Lake, MI
Faculty Sponsor:
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Type of research: Course project

Name: Austyn Lovejoy
Major: Neuroscience and Biology
Hometown: Port Wing, WI
Faculty Sponsor:
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Type of research: Course project

Name: Jack Massari
Major: Neuroscience
Hometown: Park Ridge, IL
Faculty Sponsor:
Other Sponsors:
Type of research: Course project

Name: Gabriella Urbano
Major: Neuroscience
Hometown: Mount Pleasant, WI
Faculty Sponsor:
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Type of research: Course project

Name: Ben Voiles
Major: Neuroscience and Biology
Hometown: Oconomowoc, WI
Faculty Sponsor:
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Type of research: Course project

Name: Lauren Wild
Major: Neuroscience and Religion
Hometown: Lindenhurst, IL
Faculty Sponsor:
Other Sponsors:
Type of research: Course project

Abstract

The processes carried out by the brain form the basis of learning, behavior, perception, and more. Despite how effortlessly we utilize our brains, we still know little about how and why the brain's biological processes produce such varied and specific abilities. One of the most useful ways of studying the brain’s diverse functions is to study the connections between different brain regions. A retrograde tracer can be useful in this task. Retrograde tracing is a research method used to map neuronal connections going from the axon terminal to the cell body of a neuron. This allows for the determination of the location of the origin of a pathway. The retrograde tracer is picked up at the axon terminals in the injection site and retrogradely transported along the axon to the cell body. Our tracer, Fluoro-gold, is a substance that can be visualized under a fluorescent microscope, allowing us to visually trace what regions project to a brain region of interest. Our regions of interest in this experiment were the central amygdalar nucleus, primary visual area, periventricular nucleus, nucleus accumbens, and dorsal anterior cingulate cortex. Our neuronal tracing studies demonstrate the usefulness of Fluoro-gold in better understanding connections between brain regions.

Poster file