Antibiotic resistance is rising worldwide, leading to 1.27 million deaths yearly and more difficulty treating bacterial infections. Due to the rise in resistance, the need for new antibiotics has never been greater. The Tiny Earth Project aims to discover new antibiotics produced by bacteria isolated from environmental samples from various locations across the world. Our research focuses on one species of bacteria, Bacillus weidmannii, isolated from two separate locations on the Carthage College campus in Fall 2023. By examining interactions between our isolate and non-pathogenic relatives of antibiotic-resistant pathogens, such as Mycobacterium smegmatis and Staphylococcus aureus, using an agar plug method, it was determined that B. weidmannii produces strong antimicrobial activity.  Antimicrobial activity is a bacteria’s ability to inhibit or kill another bacteria, fungi, virus, or parasite.  Strong antimicrobial activity suggests that a bacteria species has a high potential to kill another bacteria or other microbe, and this knowledge can be utilized in the creation of antibiotics.  Further research is being conducted to purify the specific antimicrobial compound produced by our bacteria. Bioinformatic analysis of the B. weidmannii genome using the antiSMASH program reveals the presence of genes involved in lasso peptide production. Research is also being conducted to induce antimicrobial activity in B. weidmannii, exposing it to various media and bacteria.  Through this experimentation, we hope to gain better knowledge of the antimicrobial power of B. weidmannii and its potential to be used in the future as an antibiotic.