Genomic Analysis of LizLemon, a Novel Mycobacteriophage from Cluster B2 Isolated from Squirrel Feces — Presentation Detail — Celebration of Scholars

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A clear statement of the problem or question you pursued, or the scholarly goal or creative theme achieved in your work.
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Additional Information

More information is available at carthage.edu/celebration-scholars/. The following are members of the Research, Scholarship, and Creativity Committee who are eager to listen to ideas and answer questions:

Jun Wang
Kim Instenes
John Kirk
Nora Nickels
Andrew Pustina
James Ripley

Genomic Analysis of LizLemon, a Novel Mycobacteriophage from Cluster B2 Isolated from Squirrel Feces

Name: Kristin Arnold
Major: Biology
Hometown:
Faculty Sponsor:
Other Sponsors:
Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Alexandra Ash
Major: Biology
Hometown:
Faculty Sponsor:
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Quenten Austin
Major: Biology
Hometown:
Faculty Sponsor:
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Kaitlyn Brahm
Major: Biology
Hometown:
Faculty Sponsor:
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Brittney Carberry
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: John Grant
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Katarina Leckie
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: athena Meder
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Alysha Newsom
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Meagan Reinecke
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Name: Kara Rognrud
Major: Biology
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Type of research: Course project
Funding: Howard Hughes Medical Institute SEA

Abstract

During the past two years, 36 novel mycobacteriophage have been isolated from local water and soil samples surrounding Carthage College. All of these phage are capable of infecting the bacterium Mycobacterium smegmatis, a non-pathogenic relative of M. tuberculosis. The genomic sequence of LizLemon, which was recently isolated from squirrel feces, revealed that it is a cluster B2 phage with extensive homology to Rosebush. The genome consists of 67,496 base pairs of DNA and contains 92 individual genes. The function of most of these genes is unknown, but many of the genes align with similar genes in other cluster B2 mycobacteriophage. Annotation and analysis of the sequence indicate that the Tapemeasure protein is 1882 amino acids in length. There is also a putative translational frameshift in gene product 67 that has not been previously identified in other B2 cluster phage. Future studies will further characterize this phage and attempt to verify the putative translational frameshift.

Poster file